Slime as a possible factor in cell clumping in Nocardia corallina.

Studies on the nutrition of Nocardia corallina.

The nutritional requirements of Streptothrix corallinus (Nocardia corallina, Breed et al., 1948) were studied by Reader (1928) who showed that growth in a medium containing glucose, ammonium sulfate and inorganic salts was stimulated by the addition of small amounts of tryptic meat broth or yeast extracts. The active principle in these additions was thought to be related to, but not identical w...

Hydrolysis of ibuprofen nitrile and ibuprofen amide and deracemisation of ibuprofen using Nocardia corallina B-276.

A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but t...

Pigment of Nocardia corallina.

Nitrile Hydratase Activity of Nocardia corallina B-276

Nitrile is a functional group that usually is transformed to amides or carboxylic acids under strong reaction conditions in acidic or basic media and high temperatures. Amides have also been prepared from nitriles at room temperature using strong oxidizing agents such as hydrogen peroxide or sodium superoxide in DMSO. On the other hand biocatalytic hydrolysis of nitriles mediated by nitrilase, ...

Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying.

The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular characteristics such as growth type in serum-soft agar, cell volume index, and clumpi...